Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy

• Main Authors: Le Li (Author), Bingjie Yu (Author), Yingji Lai (Author), Siyuan Shen (Author), Yawei Yan (Author), Guojun Dong (Author), Xiangyun Gao (Author), Yanrong Cao (Author), Caojie Ge (Author), Liqin Zhu (Author), Huan Liu (Author), Shanhui Tao (Author), Zhiang Yao (Author), Shijun Li (Author), Xiaojie Wang (Author), Qi Hui (Author)
• Format: Book
• Published: Frontiers Media S.A., 2024-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Introduction: Human basic fibroblast growth factor (hbFGF) is a highly valuable multifunctional protein that plays a crucial role in various biological processes. In this study, we aim to accomplish the scaling-up production of mature hbFGF (146aa) by implementing a high cell-density fermentation an...