Ex vivo, in situ perfusion protocol for human brain fixation compatible with microscopy, MRI techniques, and anatomical studies

• Main Authors: Ricardo Insausti (Author), Ana María Insausti (Author), Mónica Muñoz López (Author), Isidro Medina Lorenzo (Author), Maria del Mar Arroyo-Jiménez (Author), María Pilar Marcos Rabal (Author), Carlos de la Rosa-Prieto (Author), José Carlos Delgado-González (Author), Javier Montón Etxeberria (Author), Sandra Cebada-Sánchez (Author), Juan Francisco Raspeño-García (Author), María Mercedes Iñiguez de Onzoño (Author), Francisco Javier Molina Romero (Author), Ruth Benavides-Piccione (Author), Silvia Tapia-González (Author), Laura E. M. Wisse (Author), Sadhana Ravikumar (Author), David A. Wolk (Author), Javier DeFelipe (Author), Paul Yushkevich (Author), Emilio Artacho-Pérula (Author)
• Format: Book
• Published: Frontiers Media S.A., 2023-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We present a method for human brain fixation based on simultaneous perfusion of 4% paraformaldehyde through carotids after a flush with saline. The left carotid cannula is used to perfuse the body with 10% formalin, to allow further use of the body for anatomical research or teaching. The aim of our...