A Facile Method for the Removal of dsRNA Contaminant from In Vitro-Transcribed mRNA

• Main Authors: Markus Baiersdörfer (Author), Gábor Boros (Author), Hiromi Muramatsu (Author), Azita Mahiny (Author), Irena Vlatkovic (Author), Ugur Sahin (Author), Katalin Karikó (Author)
• Format: Book
• Published: Elsevier, 2019-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The increasing importance of in vitro-transcribed (IVT) mRNA for synthesizing the encoded therapeutic protein in vivo demands the manufacturing of pure mRNA products. The major contaminant in the IVT mRNA is double-stranded RNA (dsRNA), a transcriptional by-product that can be removed only by burden...