Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2
The aim of the present study was to analyze the anti-cancer effect of Orthosiphon stamineus leaf extract on MCF-7 cells. Ethyl acetate extract of the plant was eluted and pooled into 6 fractions based on their Rf values and the fractions were tested for cytotoxicity. Fraction 3 was found effective (...
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| Main Authors: | , , , , |
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| Format: | Book |
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Bangladesh Pharmacological Society,
2017-07-01T00:00:00Z.
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| Summary: | The aim of the present study was to analyze the anti-cancer effect of Orthosiphon stamineus leaf extract on MCF-7 cells. Ethyl acetate extract of the plant was eluted and pooled into 6 fractions based on their Rf values and the fractions were tested for cytotoxicity. Fraction 3 was found effective (IC50 = 28.5 µL/mL) and was subjected to LC-MS analysis which indicated the presence of biochanin, eleutherol and cinnamic acid. Further, cytotoxic effect of the fraction was confirmed in terms of nucleolin, bcl2, bax and cytochrome c expression in MCF-7 cells through RT-PCR where a marked decrease in nucleolin, bcl2 and increase in bax and cytochrome c were observed. The presence of eleutherol, a well-known anti-oxidant and anti-cancer agent, has been reported for the first time in O. stamineus. The anti-cancer activity of O. stamineus may be due to the presence of elutherol and cinnamic acid. Video Clip of Methodology: LC-MS analysis of Orthosiphon stamineus extract: 7 min 33 sec Full Screen Alternate |
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| Item Description: | 10.3329/bjp.v12i3.32337 1991-0088 |