Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2
The aim of the present study was to analyze the anti-cancer effect of Orthosiphon stamineus leaf extract on MCF-7 cells. Ethyl acetate extract of the plant was eluted and pooled into 6 fractions based on their Rf values and the fractions were tested for cytotoxicity. Fraction 3 was found effective (...
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Bangladesh Pharmacological Society,
2017-07-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_1dee51b068f74c3e9a6ec1534d10a73d | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Renuka Saravanan |e author |
| 700 | 1 | 0 | |a Brindha Pemaiah |e author |
| 700 | 1 | 0 | |a Sriram Sridharan |e author |
| 700 | 1 | 0 | |a Mahesh Narayanan |e author |
| 700 | 1 | 0 | |a Sivakumar Ramalingam |e author |
| 245 | 0 | 0 | |a Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2 |
| 260 | |b Bangladesh Pharmacological Society, |c 2017-07-01T00:00:00Z. | ||
| 500 | |a 10.3329/bjp.v12i3.32337 | ||
| 500 | |a 1991-0088 | ||
| 520 | |a The aim of the present study was to analyze the anti-cancer effect of Orthosiphon stamineus leaf extract on MCF-7 cells. Ethyl acetate extract of the plant was eluted and pooled into 6 fractions based on their Rf values and the fractions were tested for cytotoxicity. Fraction 3 was found effective (IC50 = 28.5 µL/mL) and was subjected to LC-MS analysis which indicated the presence of biochanin, eleutherol and cinnamic acid. Further, cytotoxic effect of the fraction was confirmed in terms of nucleolin, bcl2, bax and cytochrome c expression in MCF-7 cells through RT-PCR where a marked decrease in nucleolin, bcl2 and increase in bax and cytochrome c were observed. The presence of eleutherol, a well-known anti-oxidant and anti-cancer agent, has been reported for the first time in O. stamineus. The anti-cancer activity of O. stamineus may be due to the presence of elutherol and cinnamic acid. Video Clip of Methodology: LC-MS analysis of Orthosiphon stamineus extract: 7 min 33 sec Full Screen Alternate | ||
| 546 | |a EN | ||
| 690 | |a Anti-cancer | ||
| 690 | |a Cleome gynandra | ||
| 690 | |a DPPH | ||
| 690 | |a MTT | ||
| 690 | |a Palmitic acid | ||
| 690 | |a Phytol | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Bangladesh Journal of Pharmacology, Vol 12, Iss 3 (2017) | |
| 787 | 0 | |n https://www.banglajol.info/index.php/BJP/article/view/32337 | |
| 787 | 0 | |n https://doaj.org/toc/1991-0088 | |
| 856 | 4 | 1 | |u https://doaj.org/article/1dee51b068f74c3e9a6ec1534d10a73d |z Connect to this object online. |