CABE-RY: A PAM-flexible dual-mutation base editor for reliable modeling of multi-nucleotide variants

• Main Authors: Wanyu Tao (Author), Qing Liu (Author), Shisheng Huang (Author), Xin Wang (Author), Shiyuan Qu (Author), Junfan Guo (Author), Danfeng Ou (Author), Guanglei Li (Author), Yu Zhang (Author), Xiangmin Xu (Author), Xingxu Huang (Author)
• Format: Book
• Published: Elsevier, 2021-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Multi-nucleotide variants (MNVs) represent an important type of genetic variation and have biological and clinical significance. To simulate MNVs, we designed four dual-mutation base editors combining hA3A(Y130F), TadA8e(V106W), and protospacer adjacent motif (PAM)-flexible SpRY and selected cytosin...