High Efficiency Gene Correction in Hematopoietic Cells by Donor-Template-Free CRISPR/Cas9 Genome Editing

• Main Authors: Duran Sürün (Author), Joachim Schwäble (Author), Ana Tomasovic (Author), Roy Ehling (Author), Stefan Stein (Author), Nina Kurrle (Author), Harald von Melchner (Author), Frank Schnütgen (Author)
• Format: Book
• Published: Elsevier, 2018-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The CRISPR/Cas9 prokaryotic adaptive immune system and its swift repurposing for genome editing enables modification of any prespecified genomic sequence with unprecedented accuracy and efficiency, including targeted gene repair. We used the CRISPR/Cas9 system for targeted repair of patient-specific...