In Vitro Antibiofilm Activity of Fosfomycin Alone and in Combination with Other Antibiotics against Multidrug-Resistant and Extensively Drug-Resistant <i>Pseudomonas aeruginosa</i>
Background: Due to its rapid resistance development and ability to form biofilms, treatment of <i>Pseudomonas aeruginosa</i> infections is becoming more complicated by the day. Drug combinations may help reduce both resistance and biofilm formation. Methods: Using the microtiter plate as...
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| Main Authors: | , , , , |
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| Format: | Book |
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MDPI AG,
2024-06-01T00:00:00Z.
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| Summary: | Background: Due to its rapid resistance development and ability to form biofilms, treatment of <i>Pseudomonas aeruginosa</i> infections is becoming more complicated by the day. Drug combinations may help reduce both resistance and biofilm formation. Methods: Using the microtiter plate assay, we investigated the in vitro inhibition of biofilm formation and the disruption of preformed biofilms in multidrug-resistant and extensively drug-resistant clinical isolates of <i>P. aeruginosa</i> in the presence of peak plasma levels of eight antipseudomonal antibiotics alone and in combination with fosfomycin: ceftazidime, piperacillin/tazobactam, cefepime, imipenem, gentamicin, amikacin, ciprofloxacin and colistin. Results: Combination therapy was significantly superior to monotherapy in its inhibition of biofilm formation. The highest inhibition rates were observed for combinations with colistin, cefepime and ceftazidime. Conclusion: Our results support fosfomycin combination therapy as an enhanced prophylactic option. Moreover, combinations with β-lactam antibiotics and colistin demonstrated a more potent inhibition effect on biofilm formation than protein synthesis inhibitors. |
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| Item Description: | 10.3390/ph17060769 1424-8247 |