Cover Image

Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from <i>Ganoderma leucocontextum</i> Fruiting Bodies

Two low-molecular-weight polysaccharides (GLP-1 and GLP-2) were purified from <i>Ganoderma leucocontextum</i> fruiting bodies, and their physicochemical properties and antioxidant activities were investigated and compared in this study. The results showed that GLP-1 and GLP-2 were mainly...

Full description

Saved in:
Bibliographic Details
Main Authors: Xiong Gao (Author), Jiayi Qi (Author), Chi-Tang Ho (Author), Bin Li (Author), Yizhen Xie (Author), Shaodan Chen (Author), Huiping Hu (Author), Zhongzheng Chen (Author), Qingping Wu (Author)
Format: Book
Published: MDPI AG, 2021-07-01T00:00:00Z.
Subjects:
article
Online Access:Connect to this object online.
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two low-molecular-weight polysaccharides (GLP-1 and GLP-2) were purified from <i>Ganoderma leucocontextum</i> fruiting bodies, and their physicochemical properties and antioxidant activities were investigated and compared in this study. The results showed that GLP-1 and GLP-2 were mainly composed of mannose, glucose, galactose, xylose, and arabinose, with weight-average molecular weights of 6.31 and 14.07 kDa, respectively. Additionally, GLP-1 and GLP-2 had a similar chain conformation, crystal structure, and molecular surface morphology. Moreover, GLP-1 exhibited stronger antioxidant activities than GLP-2 in five different assays: 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydroxyl radical, superoxide anion radical, ferric reducing antioxidant power (FRAP), and oxygen radical antioxidant capacity (ORAC). The main linkage types of GLP-1 were found to be →4)-<i>α</i>-D-Glc<i>p</i>-(1→, →4)-<i>β</i>-D-Glc<i>p</i>-(1→, →3)-<i>β</i>-D-Glc<i>p</i>-(1→, →6)-<i>β</i>-D-Gal<i>p</i>-(1→, →6)-<i>α</i>-D-Glc<i>p</i>-(1→, →4,6)-<i>α</i>-D-Glc<i>p</i>-(1→, and Glc<i>p</i>-(1→ by methylation analysis and nuclear magnetic resonance (NMR) spectroscopy. In addition, GLP-1 could protect NIH3T3 cells against tert-butyl hydroperoxide (tBHP)-induced oxidative damage by increasing catalase (CAT) and glutathione peroxidase (GSH-Px) activities, elevating the glutathione/oxidized glutathione (GSH/GSSG) ratio, and decreasing the malondialdehyde (MDA) level. These findings indicated that GLP-1 could be explored as a potential antioxidant agent for application in functional foods.
Item Description:10.3390/antiox10071145
2076-3921

Similar Items