Viability PCR shows that non-ocular surfaces could contribute to transmission of Chlamydia trachomatis infection in trachoma.

• Main Authors: Bart Versteeg (Author), Hristina Vasileva (Author), Joanna Houghton (Author), Anna Last (Author), Oumer Shafi Abdurahman (Author), Virginia Sarah (Author), David Macleod (Author), Anthony W Solomon (Author), Martin J Holland (Author), Nicholas Thomson (Author), Matthew J Burton (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2020-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>The presence of Chlamydia trachomatis (Ct) DNA at non-ocular sites suggests that these sites may represent plausible routes of Ct transmission in trachoma. However, qPCR cannot discriminate between DNA from viable and non-viable bacteria. Here we use a propodium monoaz...