RNA-seq from archival FFPE breast cancer samples: molecular pathway fidelity and novel discovery

• Main Authors: Nathan D. Pennock (Author), Sonali Jindal (Author), Wesley Horton (Author), Duanchen Sun (Author), Jayasri Narasimhan (Author), Lucia Carbone (Author), Suzanne S. Fei (Author), Robert Searles (Author), Christina A. Harrington (Author), Julja Burchard (Author), Sheila Weinmann (Author), Pepper Schedin (Author), Zheng Xia (Author)
• Format: Book
• Published: BMC, 2019-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background Formalin-fixed, paraffin-embedded (FFPE) tissues for RNA-seq have advantages over fresh frozen tissue including abundance and availability, connection to rich clinical data, and association with patient outcomes. However, FFPE-derived RNA is highly degraded and chemically modifie...