Using a non-image-based medium-throughput assay for screening compounds targeting N-myristoylation in intracellular Leishmania amastigotes.

• Main Authors: Daniel Paape (Author), Andrew S Bell (Author), William P Heal (Author), Jennie A Hutton (Author), Robin J Leatherbarrow (Author), Edward W Tate (Author), Deborah F Smith (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2014-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We have refined a medium-throughput assay to screen hit compounds for activity against N-myristoylation in intracellular amastigotes of Leishmania donovani. Using clinically-relevant stages of wild type parasites and an Alamar blue-based detection method, parasite survival following drug treatment o...