Using a non-image-based medium-throughput assay for screening compounds targeting N-myristoylation in intracellular Leishmania amastigotes.

• Hauptverfasser: Daniel Paape (Verfasst von), Andrew S Bell (Verfasst von), William P Heal (Verfasst von), Jennie A Hutton (Verfasst von), Robin J Leatherbarrow (Verfasst von), Edward W Tate (Verfasst von), Deborah F Smith (Verfasst von)
• Format: Buch
• Veröffentlicht: Public Library of Science (PLoS), 2014-12-01T00:00:00Z.
• Schlagworte: article
• Online-Zugang: Connect to this object online.

We have refined a medium-throughput assay to screen hit compounds for activity against N-myristoylation in intracellular amastigotes of Leishmania donovani. Using clinically-relevant stages of wild type parasites and an Alamar blue-based detection method, parasite survival following drug treatment o...