gRNA Sequence Heterology Tolerance Catalyzed by CRISPR/Cas in an In Vitro Homology-Directed Repair Reaction

• Main Authors: Amanda M. Hewes (Author), Brett M. Sansbury (Author), Shaul Barth (Author), Gabi Tarcic (Author), Eric B. Kmiec (Author)
• 格式: 图书
• 出版: Elsevier, 2020-06-01T00:00:00Z.
• 主题: article
• 在线阅读: Connect to this object online.

CRISPR and associated Cas nucleases are genetic engineering tools revolutionizing innovative approaches to cancer and inherited diseases. CRISPR-directed gene editing relies heavily on proper DNA sequence alignment between the guide RNA (gRNA)/CRISPR complex and its genomic target. Accurate hybridiz...