Improvement of an HPLC method to determine urinary δ-aminolevulinic acid Aprimoramento de um método de HPLC para determinar ácido δ-aminolevulínico urinário

• Main Authors: Atecla Nunciata Lopes Alves (Author), Nairo Massakazu Sumita (Author), Alexandre Soriano Fortini (Author), Maurilio Pacheco Neto (Author), Maria Elizabete Mendes (Author), Alberto José da Silva Duarte (Author)
• Format: Book
• Published: Sociedade Brasileira de Patologia Clínica, 2010-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

OBJECTIVE: The purpose of this study was to evaluate and improve a high performance liquid chromatography (HPLC) methodology to determine urinary δ-aminolevulinic acid (ALA-U) with small volumes of sample. METHOD: The method was based on the formation of a fluorescent compound and subsequent 15-minu...