One-step synthesis of site-specific antibody-drug conjugates by reprograming IgG glycoengineering with LacNAc-based substrates

• Main Authors: Wei Shi (Author), Wanzhen Li (Author), Jianxin Zhang (Author), Tiehai Li (Author), Yakai Song (Author), Yue Zeng (Author), Qian Dong (Author), Zeng Lin (Author), Likun Gong (Author), Shuquan Fan (Author), Feng Tang (Author), Wei Huang (Author)
• Format: Book
• Published: Elsevier, 2022-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Glycosite-specific antibody‒drug conjugatess (gsADCs), harnessing Asn297 N-glycan of IgG Fc as the conjugation site for drug payloads, usually require multi-step glycoengineering with two or more enzymes, which limits the substrate diversification and complicates the preparation process. Herein, we...