The Impact of GFP Reporter Gene Transduction and Expression on Metabolomics of Placental Mesenchymal Stem Cells Determined by UHPLC-Q/TOF-MS

• Main Authors: Jinfeng Yang (Author), Nan Wang (Author), Deying Chen (Author), Jiong Yu (Author), Qiaoling Pan (Author), Dan Wang (Author), Jingqi Liu (Author), Xiaowei Shi (Author), Xiaotian Dong (Author), Hongcui Cao (Author), Liang Li (Author), Lanjuan Li (Author)
• Format: Book
• Published: Hindawi Limited, 2017-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Introduction. Green fluorescent protein (GFP) is widely used as a reporter gene in regenerative medicine research to label and track stem cells. Here, we examined whether expressing GFP gene may impact the metabolism of human placental mesenchymal stem cells (hPMSCs). Methods. The GFP gene was trans...