An Extended Multilocus Sequence Typing (MLST) Scheme for Rapid Direct Typing of Leptospira from Clinical Samples.

• Main Authors: Sabrina Weiss (Author), Angela Menezes (Author), Kate Woods (Author), Anisone Chanthongthip (Author), Sabine Dittrich (Author), Agatha Opoku-Boateng (Author), Maimuna Kimuli (Author), Victoria Chalker (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2016-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Rapid typing of Leptospira is currently impaired by requiring time consuming culture of leptospires. The objective of this study was to develop an assay that provides multilocus sequence typing (MLST) data direct from patient specimens while minimising costs for subsequent sequencing.An existing PCR...