A Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Nimbolide in Mouse Serum: Application to a Preclinical Pharmacokinetics Study

• Main Authors: Lingzhi Wang (Author), Do-Dang Khoa Phan (Author), Nicholas Syn (Author), Xiaoqiang Xiang (Author), Hongyan Song (Author), Win Lwin Thuya (Author), Shili Yang (Author), Andrea Li-Ann Wong (Author), Alan Prem Kumar (Author), Wei Peng Yong (Author), Gautam Sethi (Author), Paul Chi-Lui Ho (Author), Boon Cher Goh (Author)
• Format: Book
• Published: MDPI AG, 2018-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A sensitive and robust liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of nimbolide in mouse serum. Exemestane was used as the internal standard (IS). Here, we employed acetonitrile-based protein precipitation (PPT) for serum sample...