Self-Assembled Nanocomposite DOX/TPOR₄@CB[7]₄ for Enhanced Synergistic Photodynamic Therapy and Chemotherapy in Neuroblastoma

• Main Authors: Zhouxia Lu (Author), Xu Chen (Author), Conghui Wang (Author), Xuelian Luo (Author), Xiaohan Wu (Author), Xing Zhao (Author), Song Xiao (Author)
• Format: Book
• Published: MDPI AG, 2024-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/pharmaceutics16060822; 1999-4923
• Keywords: porphyrin; photodynamic therapy; singlet oxygen; apoptosis; Pharmacy and materia medica; RS1-441
• Source: Pharmaceutics, Vol 16, Iss 6, p 822 (2024)

DOX/TPOR₄@CB[7]₄ was synthesized via self-assembly, and its physicochemical properties and ability to generate reactive oxygen species (ROS) were evaluated. The impact of photodynamic therapy on SH-SY5Y cells was assessed using the MTT assay, while flow cytometry analysis was employed to detect cell apoptosis. Confocal laser scanning microscopy was utilized to observe the intracellular distribution of DOX/TPOR₄@CB[7]₄ in SH-SY5Y cells. Additionally, fluorescence imaging of DOX/TPOR₄@CB[7]₄ in nude mice bearing SH-SY5Y tumors and examination of the combined effects of photodynamic and chemical therapies were conducted. The incorporation of CB[7] significantly enhanced the optical properties of DOX/TPOR₄@CB[7]₄, resulting in increased ROS production and pronounced toxicity towards SH-SY5Y cells. Moreover, both the apoptotic and mortality rates exhibited significant elevation. In vivo experiments demonstrated that tumor growth inhibition was most prominent in the DOX/TPOR₄@CB[7]₄ group. π-π interactions facilitated the binding between DOX and photosensitizer TPOR, with TPOR's naphthalene hydrophilic groups encapsulated within CB[7]'s cavity through host-guest interactions with CB[7]. Therefore, CB[7] can serve as a nanocarrier to enhance the combined application of chemical therapy and photodynamic therapy, thereby significantly improving treatment efficacy against neuroblastoma tumors.