Removal of cumulus cells around 20 h after the start of in vitro maturation improves the meiotic competence of porcine oocytes via reduction in cAMP and cGMP levels

• Main Authors: Pilar FERRÉ-PUJOL (Author), Xuan Khanh NGUYEN (Author), Tomoki NAGAHARA (Author), Thi Tra Mi BUI (Author), Takuya WAKAI (Author), Hiroaki FUNAHASHI (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2019-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We examined the effect of the timing of removing cumulus cells surrounding porcine oocytes from small follicles (SFs, < 3 mm in diameter) and medium follicles (MFs; 3-6 mm in diameter) on the meiotic and developmental competence of the oocytes. Cumulus-oocyte complexes (COCs) were collected from...