Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro.

• Main Authors: Grégory Michel (Author), Bernard Ferrua (Author), Thierry Lang (Author), Madhavi P Maddugoda (Author), Patrick Munro (Author), Christelle Pomares (Author), Emmanuel Lemichez (Author), Pierre Marty (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2011-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Here we engineered transgenic Leishmania infantum that express luciferase, the objectives being to more easily monitor in real time their establishment either in BALB/c mice--the liver and spleen being mainly studied-or in vitro. Whatever stationary phase L. infantum promastigotes population--wild t...