The comparison of ZFNs, TALENs, and SpCas9 by GUIDE-seq in HPV-targeted gene therapy

• Main Authors: Zifeng Cui (Author), Hui Liu (Author), Hongfeng Zhang (Author), Zhaoyue Huang (Author), Rui Tian (Author), Lifang Li (Author), Weiwen Fan (Author), Yili Chen (Author), Lijie Chen (Author), Sen Zhang (Author), Bhudev C. Das (Author), Konstantin Severinov (Author), Inga Isabel Hitzeroth (Author), Priya Ranjan Debata (Author), Zhuang Jin (Author), Jiashuo Liu (Author), Zheying Huang (Author), Weiling Xie (Author), Hongxian Xie (Author), Bin Lang (Author), Ji Ma (Author), Haiyan Weng (Author), Xun Tian (Author), Zheng Hu (Author)
• Format: Book
• Published: Elsevier, 2021-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs), and CRISPR-associated Cas9 endonucleases are three major generations of genome editing tools. However, no parallel comparison about the efficiencies and off-target activity of the three nucleases has been reported, wh...