Double-antigen sandwich ELISA based on chimeric antigens for detection of antibodies to Trypanosoma cruzi in human sera.

• Main Authors: Natália Erdens Maron Freitas (Author), Emily Ferreira Santos (Author), Leonardo Maia Leony (Author), Ângelo Antônio Oliveira Silva (Author), Ramona Tavares Daltro (Author), Larissa de Carvalho Medrado Vasconcelos (Author), Gabriela Agra Duarte (Author), Cristiane Oliveira da Mota (Author), Edimilson Domingos Silva (Author), Paola Alejandra Fiorani Celedon (Author), Nilson Ivo Tonin Zanchin (Author), Fred Luciano Neves Santos (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2022-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>Enzyme-linked immunosorbent assays (ELISA) are generally the chosen test for Chagas disease (CD) diagnosis; however, its performance depends on the antigen preparation adsorbed to the solid phase, which may lead to false-positive results and cross-reactions. The use of...