Investigation of anomalous charge variant profile reveals discrete pH-dependent conformations and conformation-dependent charge states within the CDR3 loop of a therapeutic mAb

• Main Authors: Wenkui Lan (Author), Joseph J. Valente (Author), Andrew Ilott (Author), Naresh Chennamsetty (Author), Zhihua Liu (Author), Joseph M. Rizzo (Author), Aaron P. Yamniuk (Author), Difei Qiu (Author), Holly M. Shackman (Author), Mark S. Bolgar (Author)
• Format: Book
• Published: Taylor & Francis Group, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

During the development of a therapeutic monoclonal antibody (mAb-1), the charge variant profile obtained by pH-gradient cation exchange chromatography (CEX) contained two main peaks, each of which exhibited a unique intrinsic fluorescence profile and demonstrated inter-convertibility upon reinjectio...