Expanding the Repertoire of Target Sites for Zinc Finger Nuclease-mediated Genome Modification

• Main Authors: Kimberly A Wilson (Author), Abbye E McEwen (Author), Shondra M Pruett-Miller (Author), Jiuli Zhang (Author), Eric J Kildebeck (Author), Matthew H Porteus (Author)
• Format: Book
• Published: Elsevier, 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Recent studies have shown that zinc finger nucleases (ZFNs) are powerful reagents for making site-specific genomic modifications. The generic structure of these enzymes includes a ZF DNA-binding domain and nuclease domain (Fn) are separated by an amino acid "linker" and cut genomic DNA at...