CTLA4-Ig interferes and downregulates the proinflammatory activities of rheumatoid synovial macrophages in monoculture
Objective: CTLA4-Ig, a biologic agent employed in rheumatoid arthritis (RA) treatment, downregulates the immune response and exerts anti-inflammatory effects acting on different cells including dendritic/T cells interaction and directly on osteoclasts. We investigated the anti-inflammatory effects o...
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PAGEPress Publications,
2011-09-01T00:00:00Z.
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|---|---|---|---|
| 001 | doaj_305e711f112d44aba16a49cc4d0aeb08 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a L. Felli |e author |
| 700 | 1 | 0 | |a L. Molfetta |e author |
| 700 | 1 | 0 | |a P. Clerico |e author |
| 700 | 1 | 0 | |a P.F. Triolo |e author |
| 700 | 1 | 0 | |a B. Villaggio |e author |
| 700 | 1 | 0 | |a B. Seriolo |e author |
| 700 | 1 | 0 | |a A. Sulli |e author |
| 700 | 1 | 0 | |a P. Montagna |e author |
| 700 | 1 | 0 | |a S. Soldano |e author |
| 700 | 1 | 0 | |a R. Brizzolara |e author |
| 700 | 1 | 0 | |a M. Cutolo |e author |
| 245 | 0 | 0 | |a CTLA4-Ig interferes and downregulates the proinflammatory activities of rheumatoid synovial macrophages in monoculture |
| 260 | |b PAGEPress Publications, |c 2011-09-01T00:00:00Z. | ||
| 500 | |a 10.4081/reumatismo.2011.80 | ||
| 500 | |a 0048-7449 | ||
| 500 | |a 2240-2683 | ||
| 520 | |a Objective: CTLA4-Ig, a biologic agent employed in rheumatoid arthritis (RA) treatment, downregulates the immune response and exerts anti-inflammatory effects acting on different cells including dendritic/T cells interaction and directly on osteoclasts. We investigated the anti-inflammatory effects of CTLA4-Ig in primary monocultures of RA synovial macrophages (SM). Methods SM were obtained, from 8 RA patients (7 F, 1 M; DAS28>5.2) who underwent therapeutic arthroscopic synoviectomy and were cultured in the absence and in the presence of CTLA4-Ig at the concentration of [500 μg/ml], the most reliable dose related to the previous pharmacological clinical and experimental experiences. Inflammatory cytokine (IL-6, TNFalpha, IL-1beta) expression was evaluated by immunocytochemistry (ICC with relative image analysis), western blot (WB), and quantitative real-time polymerase chain reaction (qRT-PCR). Results: ICC analysis revealed that CTLA4-Ig treatment significantly downregulated cytokine expression (p<0.001 for IL-6, TNFalpha and IL-1beta) when compared to untreated RA SM. WB and qRT-PCR confirmed partially the data. Conclusions: CTLA4-Ig was found to exert a direct and significant anti-inflammatory effect on primary monocultures of RA SM, suggesting a therapeutic power in different phases of the disease activity. | ||
| 546 | |a EN | ||
| 546 | |a IT | ||
| 690 | |a rheumatoid arthritis, synovial macrophages, costimolatory molecules, CTLA4-Ig | ||
| 690 | |a Medicine | ||
| 690 | |a R | ||
| 690 | |a Internal medicine | ||
| 690 | |a RC31-1245 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Reumatismo, Vol 63, Iss 2, Pp 80-85 (2011) | |
| 787 | 0 | |n http://www.reumatismo.org/index.php/reuma/article/view/513 | |
| 787 | 0 | |n https://doaj.org/toc/0048-7449 | |
| 787 | 0 | |n https://doaj.org/toc/2240-2683 | |
| 856 | 4 | 1 | |u https://doaj.org/article/305e711f112d44aba16a49cc4d0aeb08 |z Connect to this object online. |