Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism

• Main Authors: Sophie Trefely (Author), Joyce Liu (Author), Katharina Huber (Author), Mary T. Doan (Author), Helen Jiang (Author), Jay Singh (Author), Eliana von Krusenstiern (Author), Anna Bostwick (Author), Peining Xu (Author), Juliane G. Bogner-Strauss (Author), Kathryn E. Wellen (Author), Nathaniel W. Snyder (Author)
• Format: Book
• Published: Elsevier, 2019-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Objective: The dynamic regulation of metabolic pathways can be monitored by stable isotope tracing. Yet, many metabolites are part of distinct processes within different subcellular compartments. Standard isotope tracing experiments relying on analyses in whole cells may not accurately reflect compa...