Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism

• मुख्य लेखकों: Sophie Trefely (लेखक), Joyce Liu (लेखक), Katharina Huber (लेखक), Mary T. Doan (लेखक), Helen Jiang (लेखक), Jay Singh (लेखक), Eliana von Krusenstiern (लेखक), Anna Bostwick (लेखक), Peining Xu (लेखक), Juliane G. Bogner-Strauss (लेखक), Kathryn E. Wellen (लेखक), Nathaniel W. Snyder (लेखक)
• स्वरूप: पुस्तक
• प्रकाशित: Elsevier, 2019-12-01T00:00:00Z.
• विषय: article
• ऑनलाइन पहुंच: Connect to this object online.

Objective: The dynamic regulation of metabolic pathways can be monitored by stable isotope tracing. Yet, many metabolites are part of distinct processes within different subcellular compartments. Standard isotope tracing experiments relying on analyses in whole cells may not accurately reflect compa...