Challenges in estimating the encapsulation efficiency of proteins in polymersomes - Which is the best method?

• Main Authors: Jorge Javier Muso-Cachumba (Author), Grace Ruiz-Lara (Author), Gisele Monteiro (Author), Carlota de Oliveira Rangel-Yagui (Author)
• Formato: Libro
• Publicado: Universidade de São Paulo, 2023-11-01T00:00:00Z.
• Subjects: article
• Acceso en liña: Connect to this object online.

Abstract Polymersomes are nanometric vesicles that can encapsulate large and hydrophilic biomolecules, such as proteins, in the aqueous core. Data in literature show large variation in encapsulation efficiency (%EE) values depending on the method used for calculation. We investigated different appro...