N-glycans released from glycoproteins using a commercial kit and comprehensively analyzed with a hypothetical database

• Main Authors: Xue Sun (Author), Lei Tao (Author), Lin Yi (Author), Yilan Ouyang (Author), Naiyu Xu (Author), Duxin Li (Author), Robert J. Linhardt (Author), Zhenqing Zhang (Author)
• Format: Book
• Published: Elsevier, 2017-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The glycosylation of proteins is responsible for their structural and functional roles in many cellular activities. This work describes a strategy that combines an efficient release, labeling and liquid chromatography-mass spectral analysis with the use of a comprehensive database to analyze N-glyca...