A simple method for HPLC retention time prediction: linear calibration using two reference substances

• Main Authors: Lei Sun (Author), Hong-yu Jin (Author), Run-tao Tian (Author), Ming-juan Wang (Author), Li-na Liu (Author), Liu-ping Ye (Author), Tian-tian Zuo (Author), Shuang-cheng Ma (Author)
• Format: Book
• Published: BMC, 2017-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background Analysis of related substances in pharmaceutical chemicals and multi-components in traditional Chinese medicines needs bulk of reference substances to identify the chromatographic peaks accurately. But the reference substances are costly. Thus, the relative retention (RR) method...