AAVS1‐targeted, stable expression of ChR2 in human brain organoids for consistent optogenetic control

• Autori principali: Soojung Hong (Autore), Juhee Lee (Autore), Yunhee Kim (Autore), Eunjee Kim (Autore), Kunyoo Shin (Autore)
• Natura: Libro
• Pubblicazione: Wiley, 2024-11-01T00:00:00Z.
• Soggetti: article
• Accesso online: Connect to this object online.

Abstract Self‐organizing brain organoids provide a promising tool for studying human development and disease. Here we created human forebrain organoids with stable and homogeneous expression of channelrhodopsin‐2 (ChR2) by generating AAVS1 safe harbor locus‐targeted, ChR2 knocked‐in human pluripoten...