Antibody Fc engineering for enhanced neonatal Fc receptor binding and prolonged circulation half-life

• Main Authors: Brian C. Mackness (Author), Julie A. Jaworski (Author), Ekaterina Boudanova (Author), Anna Park (Author), Delphine Valente (Author), Christine Mauriac (Author), Olivier Pasquier (Author), Thorsten Schmidt (Author), Mostafa Kabiri (Author), Abdullah Kandira (Author), Katarina Radošević (Author), Huawei Qiu (Author)
• Format: Book
• Published: Taylor & Francis Group, 2019-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The neonatal Fc receptor (FcRn) promotes antibody recycling through rescue from normal lysosomal degradation. The binding interaction is pH-dependent with high affinity at low pH, but not under physiological pH conditions. Here, we combined rational design and saturation mutagenesis to generate nove...