Analytical performance of a multiplex Real-Time PCR assay using TaqMan probes for quantification of Trypanosoma cruzi satellite DNA in blood samples.

• Main Authors: Tomas Duffy (Author), Carolina I Cura (Author), Juan C Ramirez (Author), Teresa Abate (Author), Nelly M Cayo (Author), Rudy Parrado (Author), Zoraida Diaz Bello (Author), Elsa Velazquez (Author), Arturo Muñoz-Calderon (Author), Natalia A Juiz (Author), Joaquín Basile (Author), Lineth Garcia (Author), Adelina Riarte (Author), Julio R Nasser (Author), Susana B Ocampo (Author), Zaida E Yadon (Author), Faustino Torrico (Author), Belkisyole Alarcón de Noya (Author), Isabela Ribeiro (Author), Alejandro G Schijman (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND: The analytical validation of sensitive, accurate and standardized Real-Time PCR methods for Trypanosoma cruzi quantification is crucial to provide a reliable laboratory tool for diagnosis of recent infections as well as for monitoring treatment efficacy. METHODS/PRINCIPAL FINDINGS: We ha...