Evaluation of the analytical and diagnostic performance of a digital droplet polymerase chain reaction (ddPCR) assay to detect Trypanosoma cruzi DNA in blood samples.

• Main Authors: Juan David Ramírez (Author), Giovanny Herrera (Author), Carolina Hernández (Author), Lissa Cruz-Saavedra (Author), Marina Muñoz (Author), Carolina Flórez (Author), Robert Butcher (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2018-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:The recent development of novel Polymerase Chain Reaction (PCR) technologies that confer theoretical advantages over quantitative PCR has considerable potential in the diagnosis of low load infections, such as Trypanosoma cruzi in the chronic phase of Chagas disease. We evaluated the util...