Development and optimization of a sensitive pseudovirus-based assay for HIV-1 neutralizing antibodies detection using A3R5 cells

• Main Authors: Qingqing Chen (Author), Jianhui Nie (Author), Weijin Huang (Author), Yanmei Jiao (Author), Lan Li (Author), Tong Zhang (Author), Juan Zhao (Author), Hao Wu (Author), Youchun Wang (Author)
• Format: Book
• Published: Taylor & Francis Group, 2018-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Sensitive assays for HIV-1 neutralizing antibody detection are urgently needed for vaccine immunogen optimization and identification of protective immune response levels. In this study, we developed an easy-to-use HIV-1 pseudovirus neutralization assay based on a human CD4+ lymphoblastoid cell line...