The mRNACalc webserver accounts for the N1-methylpseudouridine hypochromicity to enable precise nucleoside-modified mRNA quantification

• Egile Nagusiak: Esteban Finol (Egilea), Sarah E. Krul (Egilea), Sean J. Hoehn (Egilea), Xudong Lyu (Egilea), Carlos E. Crespo-Hernández (Egilea)
• Formatua: Liburua
• Argitaratua: Elsevier, 2024-06-01T00:00:00Z.
• Gaiak: article
• Sarrera elektronikoa: Connect to this object online.

Nucleoside-modified messenger RNA (mRNA) technologies necessarily incorporate N1-methylpseudouridine into the mRNA molecules to prevent the over-stimulation of cytoplasmic RNA sensors. Despite this modification, mRNA concentrations remain mostly determined through the measurement of UV absorbance at...