Influence of a 3' Terminal Ribozyme on AgoshRNA Biogenesis and Activity

• Main Authors: Elena Herrera-Carrillo (Author), Zongliang Gao (Author), Ben Berkhout (Author)
• Format: Book
• Published: Elsevier, 2019-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Short hairpin RNAs (shRNAs) can induce gene silencing via the RNA interference (RNAi) mechanism. We designed an alternative shRNA molecule with a relatively short base-paired stem that bypasses Dicer and instead is processed by the Argonaute 2 (Ago2) protein into a single guide RNA strand that effec...