Validation of the performance of a point of care molecular test for leprosy: From a simplified DNA extraction protocol to a portable qPCR.

• Asıl Yazarlar: Amanda Bertão-Santos (Yazar), Larisse da Silva Dias (Yazar), Marcelo Ribeiro-Alves (Yazar), Roberta Olmo Pinheiro (Yazar), Milton Ozório Moraes (Yazar), Fernanda Saloum de Neves Manta (Yazar), Alexandre Dias Tavares Costa (Yazar)
• Materyal Türü: Kitap
• Baskı/Yayın Bilgisi: Public Library of Science (PLoS), 2024-10-01T00:00:00Z.
• Konular: article
• Online Erişim: Connect to this object online.

The study aimed to optimize qPCR reactions using oligonucleotides from the first Brazilian molecular diagnostic kit for leprosy on a portable platform (Q3-Plus). In addition, we sought to develop a simplified protocol for DNA extraction that met point-of-care criteria. During optimization on the Q3-...