Validation of the performance of a point of care molecular test for leprosy: From a simplified DNA extraction protocol to a portable qPCR.

• Main Authors: Amanda Bertão-Santos (Author), Larisse da Silva Dias (Author), Marcelo Ribeiro-Alves (Author), Roberta Olmo Pinheiro (Author), Milton Ozório Moraes (Author), Fernanda Saloum de Neves Manta (Author), Alexandre Dias Tavares Costa (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2024-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The study aimed to optimize qPCR reactions using oligonucleotides from the first Brazilian molecular diagnostic kit for leprosy on a portable platform (Q3-Plus). In addition, we sought to develop a simplified protocol for DNA extraction that met point-of-care criteria. During optimization on the Q3-...