Validation of the performance of a point of care molecular test for leprosy: From a simplified DNA extraction protocol to a portable qPCR.

• Päätekijät: Amanda Bertão-Santos (Tekijä), Larisse da Silva Dias (Tekijä), Marcelo Ribeiro-Alves (Tekijä), Roberta Olmo Pinheiro (Tekijä), Milton Ozório Moraes (Tekijä), Fernanda Saloum de Neves Manta (Tekijä), Alexandre Dias Tavares Costa (Tekijä)
• Aineistotyyppi: Kirja
• Julkaistu: Public Library of Science (PLoS), 2024-10-01T00:00:00Z.
• Aiheet: article
• Linkit: Connect to this object online.

The study aimed to optimize qPCR reactions using oligonucleotides from the first Brazilian molecular diagnostic kit for leprosy on a portable platform (Q3-Plus). In addition, we sought to develop a simplified protocol for DNA extraction that met point-of-care criteria. During optimization on the Q3-...