Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype.

• Main Authors: Benjamin Lopez-Jimena (Author), Michaël Bekaert (Author), Mohammed Bakheit (Author), Sieghard Frischmann (Author), Pranav Patel (Author), Etienne Simon-Loriere (Author), Louis Lambrechts (Author), Veasna Duong (Author), Philippe Dussart (Author), Graham Harold (Author), Cheikh Fall (Author), Oumar Faye (Author), Amadou Alpha Sall (Author), Manfred Weidmann (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2018-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

4 one-step, real-time, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed for the detection of dengue virus (DENV) serotypes by considering 2,056 full genome DENV sequences. DENV1 and DENV2 RT-LAMP assays were validated with 31 blood and 11 serum samples fro...