Dissecting the active site of the collagenolytic cathepsin L3 protease of the invasive stage of Fasciola hepatica.

• Main Authors: Ileana Corvo (Author), Anthony J O'Donoghue (Author), Lucía Pastro (Author), Natalia Pi-Denis (Author), Alegra Eroy-Reveles (Author), Leda Roche (Author), James H McKerrow (Author), John P Dalton (Author), Charles S Craik (Author), Conor R Caffrey (Author), José F Tort (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A family of secreted cathepsin L proteases with differential activities is essential for host colonization and survival in the parasitic flatworm Fasciola hepatica. While the blood feeding adult secretes predominantly FheCL1, an enzyme with a strong preference for Leu at the S2 pocket of the active...