Antibiotic Resistance in Shiga Toxigenic <i>Escherichia coli</i> Isolates from Surface Waters and Sediments in a Mixed Use Urban Agricultural Landscape

• Main Authors: Yvonne Ma (Author), Jessica Chen (Author), Karen Fong (Author), Stephanie Nadya (Author), Kevin Allen (Author), Chad Laing (Author), Kim Ziebell (Author), Ed Topp (Author), Laura M. Carroll (Author), Martin Wiedmann (Author), Pascal Delaquis (Author), Siyun Wang (Author)
• Format: Book
• Published: MDPI AG, 2021-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Antibiotic resistance (AR) phenotypes and acquired resistance determinants (ARDs) detected by in silico analysis of genome sequences were examined in 55 Shiga toxin-producing <i>Escherichia coli</i> (STEC) isolates representing diverse serotypes recovered from surfaces waters and sediments in a mixed use urban/agricultural landscape in British Columbia, Canada. The isolates displayed decreased susceptibility to florfenicol (65.5%), chloramphenicol (7.3%), tetracycline (52.7%), ampicillin (49.1%), streptomycin (34.5%), kanamycin (20.0%), gentamycin (10.9%), amikacin (1.8%), amoxicillin/clavulanic acid (21.8%), ceftiofur (18.2%), ceftriaxone (3.6%), trimethoprim-sulfamethoxazole (12.7%), and cefoxitin (3.6%). All surface water and sediment isolates were susceptible to ciprofloxacin, nalidixic acid, ertapenem, imipenem and meropenem. Eight isolates (14.6%) were multidrug resistant. ARDs conferring resistance to phenicols (<i>floR</i>), trimethoprim (<i>dfrA</i>), sulfonamides (<i>sul1/2</i>), tetracyclines (<i>tetA/B</i>), and aminoglycosides (<i>aadA</i> and <i>aph</i>) were detected. Additionally, narrow-spectrum β-lactamase <i>bla</i>TEM-1b and extended-spectrum AmpC β-lactamase (cephalosporinase) <i>bla</i>CMY-2 were detected in the genomes, as were replicons from plasmid incompatibility groups IncFII, IncB/O/K/Z, IncQ1, IncX1, IncY and Col156. A comparison with surveillance data revealed that AR phenotypes and ARDs were comparable to those reported in generic <i>E. coli</i> from food animals. Aquatic environments in the region are potential reservoirs for the maintenance and transmission of antibiotic resistant STEC, associated ARDs and their plasmids.

Item Description

10.3390/antibiotics10030237
2079-6382