CRISPR-Cas9-directed gene tagging using a single integrase-defective lentiviral vector carrying a transposase-based Cas9 off switch

• Main Authors: Emil Aagaard Thomsen (Author), Kristian Alsbjerg Skipper (Author), Sofie Andersen (Author), Didde Haslund (Author), Thomas Wisbech Skov (Author), Jacob Giehm Mikkelsen (Author)
• Format: Book
• Published: Elsevier, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Locus-directed DNA cleavage induced by the CRISPR-Cas9 system triggers DNA repair mechanisms allowing gene repair or targeted insertion of foreign DNA. For gene insertion to be successful, availability of a homologous donor template needs to be timed with cleavage of the DNA by the Cas9 endonuclease...