Effect of L-Glutathione Treatment during Somatic Cell Nuclear Transfer Procedures on the Subsequent Embryonic Development and DNA Methylation Status of Cloned Bovine Embryos

• Main Authors: Hyo-Kyung Bae (Author), Nam-Sik Yoon (Author), In-Sun Hwang (Author), Choon-Keun Park (Author), Boo-Keun Yang (Author), Hee-Tae Cheong (Author)
• Format: Book
• Published: The Korean Society of Animal Reproduction and Biotechnology, 2014-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We investigate the effect of L-glutathione (GSH), an antioxidant, treatment during the somatic cell nuclear transfer (SCNT) procedures on the in vitro development and DNA methylation status of bovine SCNT embryos. Bovine in vitro matured (IVM) oocytes were enucleated and electrofused with a donor ce...