PCR for detection of Leishmania donovani from microscopically negative tissue smears of suspected patients in Gondar, Ethiopia.

• Main Authors: Roma Melkamu (Author), Nega Berhane (Author), Bart K M Jacobs (Author), Rezika Mohammed (Author), Mekibib Kassa (Author), Arega Yeshanew (Author), Helina Fikre (Author), Saba Atnafu (Author), Saskia van Henten (Author), Johan van Griensven (Author), Myrthe Pareyn (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2023-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>As untreated visceral leishmaniasis (VL) is fatal, reliable diagnostics are pivotal for accurate treatment allocation. The current diagnostic algorithm for VL in Ethiopia, which is based on the rK39 rapid diagnostic test and microscopy of tissue smears, lacks sensitivi...