Targeting of cholera toxin A (ctxA) gene by zinc finger nuclease: pitfalls of using gene editing tools in prokaryotes

• Main Authors: Nafiseh Hosseini (Author), Hossein Khanahmad (Author), Bahram Nasr Esfahani (Author), Mojgan Bandehpour (Author), Laleh Shariati (Author), Nushin Zahedi (Author), Bahram Kazemi (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Background and purpose: The study was launched to use zinc finger nuclease (ZFN) technology to disrupt the cholera toxin gene (ctxA) for inhibiting CT toxin production in Vibrio cholera (V. cholera). Experimental approach: An engineered ZFN was designed to target the catalytic site of the ctxA gene....