Deep mutational engineering of broadly-neutralizing nanobodies accommodating SARS-CoV-1 and 2 antigenic drift

• Main Authors: Adrien Laroche (Author), Maria Lucia Orsini Delgado (Author), Benjamin Chalopin (Author), Philippe Cuniasse (Author), Steven Dubois (Author), Raphaël Sierocki (Author), Fabrice Gallais (Author), Stéphanie Debroas (Author), Laurent Bellanger (Author), Stéphanie Simon (Author), Bernard Maillère (Author), Hervé Nozach (Author)
• Format: Book
• Published: Taylor & Francis Group, 2022-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Here, we report the molecular engineering of nanobodies that bind with picomolar affinity to both SARS-CoV-1 and SARS-CoV-2 receptor-binding domains (RBD) and are highly neutralizing. We applied deep mutational engineering to VHH72, a nanobody initially specific for SARS-CoV-1 RBD with little cross-...