qPCR-High resolution melt analysis for drug susceptibility testing of Mycobacterium leprae directly from clinical specimens of leprosy patients.

• Main Authors: Sergio Araujo (Author), Luiz Ricardo Goulart (Author), Richard W Truman (Author), Isabela Maria B Goulart (Author), Varalakshmi Vissa (Author), Wei Li (Author), Masanori Matsuoka (Author), Philip Suffys (Author), Amanda B Fontes (Author), Patricia S Rosa (Author), David M Scollard (Author), Diana L Williams (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2017-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:Real-Time PCR-High Resolution Melting (qPCR-HRM) analysis has been recently described for rapid drug susceptibility testing (DST) of Mycobacterium leprae. The purpose of the current study was to further evaluate the validity, reliability, and accuracy of this assay for M. leprae DST in cl...