Effective Targeted Gene Knockdown in Mammalian Cells Using the piggyBac Transposase-based Delivery System

• मुख्य लेखकों: Jesse B Owens (लेखक), Juanita Mathews (लेखक), Philip Davy (लेखक), Ilko Stoytchev (लेखक), Stefan Moisyadi (लेखक), Rich Allsopp (लेखक)
• स्वरूप: पुस्तक
• प्रकाशित: Elsevier, 2013-01-01T00:00:00Z.
• विषय: article
• ऑनलाइन पहुंच: Connect to this object online.

Nonviral gene delivery systems are rapidly becoming a desirable and applicable method to overexpress genes in various types of cells. We have recently developed a piggyBac transposase-based, helper-independent and self-inactivating delivery system (pmGENIE-3) capable of high-efficiency transfection...