Effective Targeted Gene Knockdown in Mammalian Cells Using the piggyBac Transposase-based Delivery System

• Main Authors: Jesse B Owens (Author), Juanita Mathews (Author), Philip Davy (Author), Ilko Stoytchev (Author), Stefan Moisyadi (Author), Rich Allsopp (Author)
• Format: Book
• Published: Elsevier, 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Nonviral gene delivery systems are rapidly becoming a desirable and applicable method to overexpress genes in various types of cells. We have recently developed a piggyBac transposase-based, helper-independent and self-inactivating delivery system (pmGENIE-3) capable of high-efficiency transfection...