Effective Targeted Gene Knockdown in Mammalian Cells Using the piggyBac Transposase-based Delivery System

• Autori principali: Jesse B Owens (Autore), Juanita Mathews (Autore), Philip Davy (Autore), Ilko Stoytchev (Autore), Stefan Moisyadi (Autore), Rich Allsopp (Autore)
• Natura: Libro
• Pubblicazione: Elsevier, 2013-01-01T00:00:00Z.
• Soggetti: article
• Accesso online: Connect to this object online.

Nonviral gene delivery systems are rapidly becoming a desirable and applicable method to overexpress genes in various types of cells. We have recently developed a piggyBac transposase-based, helper-independent and self-inactivating delivery system (pmGENIE-3) capable of high-efficiency transfection...