A New Practice to Monitor the Fabrication Process of Fab-Targeting Ligands from Bevacizumab by LC-MS: Preparation and Analytical Characterization

• المؤلفون الرئيسيون: Franck Marquet (مؤلف), Valentina D'Atri (مؤلف), Davy Guillarme (مؤلف), Gerrit Borchard (مؤلف)
• التنسيق: كتاب
• منشور في: MDPI AG, 2022-01-01T00:00:00Z.
• الموضوعات: article
• الوصول للمادة أونلاين: Connect to this object online.

The objective of this study was to qualitatively evaluate a Fab-targeting ligand preparation containing free thiol groups in the hinge region by using bevacizumab as a model. The evaluation focused on the purification of fragments through a nonaffinity-based process using a centrifugal ultrafiltrati...